The production of 2H-labeled amino acids by a new mutant of RuMP fucultative methylotroph Вrevibacte...

For our studies we have used a new non-traditional producer of phenylalanine: a leucine auxotroph of the facultative methylotrophic bacterium

The production of 2H-labeled amino acids by a new mutant of RuMP fucultative methylotroph Вrevibacte...

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> m/z with 336.4 (M+)). The label was distributed uniformely among the amino acid molecules, in experiment (e) the enrichment of 2H-labeled amino acids was nevertheless less than we estimated theoretically, because Leu was added in growth medium in protonated form. This effect should be seriously scrutinised before the applying this mutant for the preparation of 2H-labeled amino acids.

 

 

 

References

1. Antony C (1982) Bacterial Oxidation of Methane and Methanol, The Biochemistry of Methylotrophs, 2 nd edn. Academic Press, London, p 78

2. Boer L de, Harder W, Dijkhuizen L (1988) Phenylalanine and Tyrosine Metabolism in the Facultative Methylotroph Nocardia sp. 239. Arch Microbiol 149: 459-465

3. Conn EE (1986) Recent Advances in Phytochemistry, The Shikimic Acid Pathway, 2 nd edn. Plenum Press, New York, p 20

4. Cox J, Kyli D, Radmer (1988) Stable Isotope Labeled Biochemicals from Microalgae. Trends Biotechnol 6: 279-282

5. Daboll HF, Crespi HL, Katz JJ (1962) Mass Cultivation of Algae in Pure Heavy Water. Biotechnol and Bioengineering 4: 281-297

6. Daub GM (1979) Syntheses with Stable Isotopes. In: Daub GM Stable Isotopes. Proceedings of the 3d Inter Conf, 1979, Academic Press, NY 1979, pp 31-44

7. Devenyi T, Gergely J (1976) Amino Acids, peptides and proteins, 3 d edn. Mir Publishers, Moscow, p 281

8. Dijkhuizen L (1996) Metabolic Regulation in the Actinomycete Amycolatopsi Methanolica, a Facultative Methylotroph Employing the RuMP Cycle for Formaldehyde Assimilation. Microbial Growth on C1 Compounds Kluwer Academic Publishers, London, pp 9-15

9. Faleev NG, Ruvinov SB, Saporovskaya MB, Belikov VM, Zakomyrdina LN (1989) Preparation of -Deuterated Amino Acids by E. coli Cells Containing Tryptophanase. Izv Akad Nauk USSR Ser Khim (in Russian) 10: 2341-2343

10. Greenstein JP, Winitz M (1976) Chemistry of amino acids, 3 d edn. Mir Publishers, Moscow, p 396

Karnaukhova EN, Reshetova OS, Semenov SY, Skladnev DA, Tsygankov YD (1994) 2H- and 13C-Labeled Amino Acids Generated by Obligate Methylotrophs Biosynthesis and MS Monitoring. Amino Acids 6: 165-176

11. Kletsova LV, Chibisova ES, Tsygankov YD (1988) Mutants of the Oligate Methylotroph Methylobacillus flagellatum KT Defective in Genes of the Ribulose Monophosphate Cycle of Formaldehyde fixation. Arch Microbiol 149: 441-446

12. LeMaster DM (1990) Deuterium Labeling in NMR Structural Analysis of Larger Proteins. Quart Revs Biophys 23: 133-174

13. Miller JH (1976) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory Cold Spring Harbor, New York p 393

14. Mosin OV, Skladnev DA, Tsygankov YD Russian Patent 93055824/13 (Nov. 17, 1995)

15. Nesvera J, Patek M, Hochmannova J, Chibisova E, Serebrijski I, Tsygankov Y, Netrusov A (1991) Transformation of a New Gram-Positive Methylotroph, Brevibacterium methylicum, by plasmid DNA. Appl Microbiol Biotechnol 35: 777-780

16. Umbarger HE (1978) Amino Acid Biosynthesis and its regulation. Ann Rev Biochim 47, 533-606

17. Walker TE, Matheny C (1986) An Efficient Chemomicrobiological Synthesis of Stable Isotope-Labeled L-Tyrosine and L-Phenylalanine. J Org Chem 51: 1175-1179.

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