The production of 2H-labeled amino acids by a new mutant of RuMP fucultative methylotroph Вrevibacte...

For our studies we have used a new non-traditional producer of phenylalanine: a leucine auxotroph of the facultative methylotrophic bacterium

The production of 2H-labeled amino acids by a new mutant of RuMP fucultative methylotroph Вrevibacte...


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> m/z with 336.4 (M+)). The label was distributed uniformely among the amino acid molecules, in experiment (e) the enrichment of 2H-labeled amino acids was nevertheless less than we estimated theoretically, because Leu was added in growth medium in protonated form. This effect should be seriously scrutinised before the applying this mutant for the preparation of 2H-labeled amino acids.





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